IJAST

Phylogenetic Polymorphisms of Gambian and Togolese Rural Chickens Assessed with Mitochondrial DNA

© 2024 by IJACT

Volume 2 Issue 2

Year of Publication : 2024

Author : Tor, N.E.T., Adebambo, A.O., Ajibike, A.B., Mendy, E.W., Oladejo, O., Adeola, A.C.

DOI : 10.56472/25839233/IJAST-V2I2P101

Citation :

Tor, N.E.T., Adebambo, A.O., Ajibike, A.B., Mendy, E.W., Oladejo, O., Adeola, A.C., 2024. "Phylogenetic Polymorphisms of Gambian and Togolese Rural Chickens Assessed with Mitochondrial DNA" ESP International Journal of Advancements In Science & Technology (ESP- IJAST)  Volume 2, Issue 2 : 1-6

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Abstract :

Gambia and Togo are geographically, two of the smallest of the sixteen-member block of Economic Community of West African States (ECOWAS). Like the rest of Africa, not much is known about the phylogenetic variations, evolution and molecular (DNA) signatures of the indigenous chicken populations of the two countries. To achieve this, genomic DNA was extracted from a total of 40 village chickens of both countries using FTA cards, following standard protocols. Sanger sequencing of the D-loop amplicons of the mitochondrial DNA was outsourced to StabVida Laboratory, Portugal, using specific primers. Multiple alignments of a final length of 920bp of the sequences with a reference sequence reveals 8 SNPs. Reconstruction of evolutionary origins using MEGA 7 and NETWORK 4.6 matrix suggests that Gambian and Togolese chickens are most likely of south Asian descent. Molecular indices revealed that the chickens in the two populations are of comparatively lower genetic diversity, nonetheless, the Gambian population was slightly more diverse than the Togolese. This finding may be employed in the genetic conservation strategies of chickens in the two countries.

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Keywords :

Chicken, Gambia, Mitochondrial DNA, Phylogenetics, Togo.